Understanding HPLC in Peptide Purity Testing
How High-Performance Liquid Chromatography is used to verify research peptide purity.
High-Performance Liquid Chromatography (HPLC) separates compounds within a sample based on their interaction with a stationary phase, allowing analysts to quantify the relative purity of a peptide lot. Peaks in an HPLC chromatogram correspond to distinct chemical species; the area under the primary peak, relative to total peak area, is commonly reported as a purity percentage. This article explains how to read a chromatogram, what retention time indicates, and why independent third-party testing strengthens confidence in a Certificate of Analysis.
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